FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999527

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999527
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23486543
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC23850.018.3013824
GACCGCG24250.018.1848355
ACCGCGT24400.017.7964136
CCGCGTT40550.015.8142171
CAGACCG28950.015.6998843
AACGCTA91650.013.20844823
ACGCTAC91750.013.19304124
CGCGTTC46700.012.9607182
TCGTTAC13950.012.903254544
CCGGCAT23800.012.6683921
CGCTACC97100.012.5355425
GCCGTAT8450.012.5151151
TTCACGC106850.012.4024017
CCGGCTT104300.012.31815052
GCCGGCT96500.012.1946131
GCCTACG16000.012.09326310
CGCGTTT9500.012.0792581
TCACGCT107550.011.9660458
GAAAACG102450.011.83803820
AAAACGC102450.011.81607521
Produced by FastQC (version 0.11.5)