FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999553

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999553
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30028574
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG20500.014.15585226
ATATAGC37950.011.2679676
CGTAACG9700.010.89801342
GTCGTTC29650.010.54630729
TCTATAG152400.010.4897593
TGTCGTT29800.010.26686928
TTCTATA168850.010.0536052
GGCGTAG35350.09.99141734
CGTAGGG35800.09.86579336
GCGTAGG35550.09.80856135
TTTCTAT183300.09.6171671
CGTGGCG27600.09.5438741
CCGCGTT12000.09.37644313
TCGATAA28100.09.36739416
CGACCCG27200.09.2672916
TATAGCT47750.09.1912617
CGATAAG29050.09.13855117
GCGTTCG10650.09.08451115
GACGCAA27600.09.04568944
TTGTCGT34150.09.02486827
Produced by FastQC (version 0.11.5)