FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999558

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999558
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15799205
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT2134841.3512325461945711No Hit
CCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAA270970.17150862970636813No Hit
CAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCA204230.12926599787774132No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGACCG295900.035.002613
TCAGACC301200.034.9294322
CTCAGAC309250.034.847461
AGACCGC299500.034.53684
GACCGCG312650.033.090655
ACCGCGT316000.032.7378776
CCGCGTT347450.029.7810337
CGCGTTC359050.028.7998988
GCGTTCT375300.027.6067719
CGTTCTC394350.026.35874210
GAACGAT391850.025.88013845
ATACGGA397400.025.66453633
AATACGG397400.025.66453632
TACGGAG396550.025.64003234
CAATACG401450.025.42795831
CCCAATA405850.025.25761429
CCTCTCA405450.025.20444319
AGAACGA403300.025.16171844
ACTCCCC411050.024.99806425
GTTCTCT417800.024.99776811
Produced by FastQC (version 0.11.5)