FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999565

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999565
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37888475
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA380760.10049493942419166No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG113950.012.32282738
CGTGGCG72200.012.2165411
GGCATAG115000.011.89757335
GTCACTC171700.011.8706418
GGGAGGT192200.011.8463533
GGTCACT173900.011.8106947
CGACCCG74850.011.6317536
ACTCTCC177900.011.58401911
GGCGACC77450.011.2126264
TGGCGAC78800.011.1922493
GTGGCGA77800.011.1625382
GTTGTCG19250.011.10530926
AGCTTAG123150.010.99608745
AGGTCAC200450.010.8192456
ACGCGCG16000.010.5458520
TCTATAG204600.010.5125913
TTTCTAT217950.010.4167841
GCGACCC84550.010.37715155
TTCTATA215550.010.3438182
GGAGGTC216200.010.343614
Produced by FastQC (version 0.11.5)