FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999586

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999586
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19803682
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT759590.38355998647120265No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGACCG134350.030.096283
AGACCGC135950.029.5931194
GACCGCG139250.028.8264475
ACCGCGT144950.027.8155846
TCAGACC149450.027.630982
CTCAGAC178200.024.8570921
CCGCGTT161950.024.8401957
CGCGTTC170650.023.5473758
GCGTTCT187300.021.5261029
TTCGACG9500.020.1309266
CGGTATA14100.019.62712320
CGTTCTC216650.018.95262310
GAACGAT211200.018.33498845
AATACGG213550.018.28008532
ATACGGA215100.018.1169833
TACGGAG214450.017.9620134
CAATACG223000.017.60633331
AGAACGA225950.017.15791144
CCCAATA230650.017.13939529
ACGGAGA225900.017.11134535
Produced by FastQC (version 0.11.5)