FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999587

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999587
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19803682
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGG213430.10777288789024184No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA213070.107591103512973No Hit
CTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAAC198580.10027428232790246No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG12450.016.08202726
ATATAGC23850.012.6439476
TGTCGTT16300.012.14561828
GTCGTTC17100.011.577167529
ATAGGGG55700.010.82309438
AAGCCGG104600.010.7099445
TTCTATA125050.010.4596972
TCTATAG119650.010.3491113
GGCATAG59100.010.23946535
AGGGATC49500.010.2330242
GGAGGTC94650.010.2045814
GGGAGGT96750.010.1923933
TATAGCT29700.010.0023087
TTTCTAT134700.09.9445171
GGTCACT90400.09.9082587
ACTCTCC93250.09.89604511
CGTAGGG31550.09.83978537
GTCACTC91550.09.8340068
GCGTTCG12200.09.77486715
TCGTTCC20750.09.75753630
Produced by FastQC (version 0.11.5)