FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999596

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999596
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43831969
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA594790.13569775977894125No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG548500.12513697479572503No Hit
CTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAAC454350.1036572187756384No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGGT281150.016.6688423
GGAGGTC281400.016.3932764
GAGGTCA310150.014.9950425
ATAGGGG208700.014.29281238
GGTCACT320700.014.0483887
GTCACTC327550.013.9137458
GGCATAG213850.013.9115235
AGGTCAC338300.013.5687516
AGGGATC124850.013.3776382
AGCTTAG225850.013.0115945
GTTGTCG26700.012.87391326
ACTCTCC359700.012.7575111
TGGGAGG250550.012.6593612
CACTCTC368000.012.4676910
TGGCATA242700.012.4216134
TCACTCT386100.012.1615119
GAGCTTA245150.012.09678544
CTGGGAG310500.011.5696361
GCGTGAA311350.011.32980940
CAGGGAT166750.011.142241
Produced by FastQC (version 0.11.5)