FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999603

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999603
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47130277
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAAC1185210.2514752883799092No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA820750.1741449556937677No Hit
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGG615740.13064637833552303No Hit
CAGGGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAG495210.10507258423284888No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG473110.100383454143501No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG40550.022.359126
TGTCGTT47450.019.15503728
ATATAGC53450.018.9060826
AGGGATC153850.017.837012
GTCGTTC52400.017.3453629
TTGTCGT57550.016.14513227
AATATAG76100.015.4131385
TCGTTCC61050.015.14542930
TATAGCT66750.015.0377637
CAGGGAT214950.014.9444461
GCTAAGT63300.014.68056314
GGGATCA188250.014.6738133
CAATATA74200.014.5943644
CGTTCCC68700.013.45891531
TACAATA79800.013.174372
GGATCAT209900.012.8495654
CGTCGAT22950.012.74585612
ATAGCTC76000.012.49016139
GCGTAGG73900.012.42016235
ATAGGGG144600.012.33642438
Produced by FastQC (version 0.11.5)