FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999604

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999604
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50997412
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT2093030.41041886596127664No Hit
CCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAA565100.11080954460983236No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGACCG358600.031.1122233
AGACCGC360900.030.795394
GACCGCG367350.030.1863175
ACCGCGT376700.029.5468256
TCAGACC404800.028.2951562
CTCAGAC450650.026.8429151
CCGCGTT454600.024.4885037
CGCGTTC486200.022.8597038
GCGTTCT533850.020.8993829
CGTTCTC603400.018.85594710
AATACGG641250.017.04915232
ATACGGA643450.016.9873833
TACGGAG644200.016.83838834
GAACGAT645800.016.66460445
CAATACG677050.016.36021231
CCCAATA680750.016.3141829
AGAACGA671400.016.11632744
ACGGAGA677650.016.05373435
CCTCTCA702500.015.971827519
CCCCAAT698000.015.94966528
Produced by FastQC (version 0.11.5)