FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999612

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999612
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54933919
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT4378670.7970794874474548No Hit
CCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAA665240.12109822348556636No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGACCG729350.032.065483
AGACCGC728250.032.0516554
TCAGACC754700.031.8190922
GACCGCG732100.031.6432915
ACCGCGT746350.031.2498136
CTCAGAC794300.031.1722621
CCGCGTT837050.027.8450977
CGCGTTC874000.026.660178
GCGTTCT932600.025.0042789
CGTTCTC1009400.023.74825510
GAACGAT1045250.021.7647445
AATACGG1064400.021.44293832
CCTCTCA1103100.021.42249919
ATACGGA1068200.021.36262133
TACGGAG1055200.021.35713834
GTTCTCT1132550.021.25935611
AGAACGA1088150.020.92928344
ACGGAGA1077750.020.86233535
ACTCCCC1132700.020.85486825
CAATACG1112300.020.80258631
Produced by FastQC (version 0.11.5)