FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999629

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999629
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44151877
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCT1074670.24340301545956924No Hit
CTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAAC907520.20554505530987957No Hit
CTTCTATCCCAGCGACATCGCCGTGGAGTGGGAGAGCAATGGGCAGCCGGA794370.17991760576792692No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA698610.15822883362353993No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTG666500.15095620963068004No Hit
CAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGC500680.11339948242744018No Hit
CTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGG495830.11230100138211564No Hit
CTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAA473690.10728649203294346No Hit
CACAGGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGG458440.10383250524094367No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTTAGG68050.023.0578214
GTCAGGA287950.021.5187031
GTTGTCG25400.018.8659226
CAGTTAG85500.018.613883
GTTAGGA91400.017.044255
TTAGGAC95750.016.4559756
AGCGCTC113100.016.2586489
TCAGTTA104500.015.9818442
CGCTCTC118250.015.62493611
AGGGATC143050.015.5456312
GGGATCA140900.015.2572393
GCATTCG49750.015.2482633
CAGCGCT126800.015.05254658
ATATAGC35000.014.9848436
TTAGCCC50650.014.9757882
GTCGTTC31800.014.64460729
TGTCGTT32200.014.46265428
GCAGCGC132150.014.3754817
CGCGTTC28500.014.36850914
TAGGACC112450.014.0515687
Produced by FastQC (version 0.11.5)