FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999631

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999631
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34079083
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA558830.16398035123186852No Hit
CTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAAC432440.1268930857089083No Hit
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGG395780.1161357540048833No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG26150.018.06837726
TGTCGTT36200.013.36248928
ATATAGC42150.013.1863476
AGGGATC103850.012.8505742
GTCGTTC36650.012.70711129
TTGTCGT39900.012.57470327
GGAGGTC148200.012.2850394
GCTAAGT43500.011.63803114
GGGAGGT163100.011.5766853
CAGGGAT139200.011.187661
TCGTTCC42300.011.16915830
TTTCTAT320850.011.1523771
TTCTATA319050.011.0742722
TATAGCT53450.010.9879827
TCTATAG312250.010.8181873
GGGATCA130700.010.67563
GAGGTCA175950.010.6291575
ATAGGGG117600.010.38803538
AGGTCAC175250.010.336256
GTCACTC166300.010.2292978
Produced by FastQC (version 0.11.5)