FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999648

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999648
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36259763
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG482210.13298763149665374No Hit
CTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAAC433430.11953470297089365No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA390460.10768410152046498No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG34450.020.50562326
ATATAGC43650.019.2328176
TGTCGTT41600.017.52197328
GTCGTTC42950.016.86627829
TATAGCT52700.015.9727137
GCTAAGT47150.015.89105114
TCGTTCC47350.015.53619830
AGGGATC106450.014.5085062
ATAGGGG173450.014.434545538
GGGAGGT277300.014.3467163
CAATATA65650.014.1297824
GGAGGTC281200.014.0759124
GTCACTC276900.013.9472538
AGGTCAC286750.013.727926
GGCATAG185050.013.70060735
AATATAG72950.013.5489885
ACTCTCC290000.013.47801911
CGTTCCC53400.013.4389131
GAGGTCA295650.013.4030125
TACAATA66200.013.3653022
Produced by FastQC (version 0.11.5)