FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999659

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999659
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31047596
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGCG91650.014.201771
GTGGCGA93000.013.8509352
TGGCGAC99450.013.2025923
GTTGTCG17350.013.09470526
CGTCGAT20400.012.90625712
GCGTTCG19450.012.84036215
GGCGACC102750.012.8217624
CGACCCG106200.012.80254656
GCGTCGA21550.012.74058411
CCGCGTT19650.012.59661113
CGCGTTC19800.012.50065814
AACTAAC129750.012.3962637
CGTTCGC21150.012.12679416
ATATAGC28850.011.8599186
GCGACCC113250.011.8514055
ACTAACC136850.011.7364238
TGCGTCG22700.011.69923410
TTCTATA168800.011.6601722
TTTCTAT176000.011.5153451
TCTATAG164600.011.4933033
Produced by FastQC (version 0.11.5)