FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999668

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999668
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49175114
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT3233560.6575602448018728No Hit
CCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAA594990.12099412723272995No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGACCG511250.033.4493033
AGACCGC514700.033.137664
TCAGACC539850.032.8728452
GACCGCG518450.032.8150755
ACCGCGT529250.032.2366836
CTCAGAC603400.030.6477681
CCGCGTT611100.027.9302717
CGCGTTC648300.026.3318
GCGTTCT699850.024.449369
CGTTCTC780750.022.55283210
AATACGG837450.020.32480432
ATACGGA843250.020.19843133
TACGGAG839700.020.1179234
CAATACG858550.020.00350231
TTCGACG18450.019.8774856
GTTCTCT904200.019.76484111
CCCAATA882250.019.70586829
GAACGAT869350.019.62569245
ACGGAGA862100.019.61354435
CCTCTCA855250.019.51208119
Produced by FastQC (version 0.11.5)