FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999669

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999669
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49175114
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA1041600.21181445558011314No Hit
CTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAAC941420.19144236249254043No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG919540.1869929574540488No Hit
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGG657020.13360823118783213No Hit
GTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGT635420.1292157655191201No Hit
CCGGGAGGTCACTCTCCCCGGGCTCTGTCCAAGTGGCGTAGGGGAGCATAG603370.12269824122827656No Hit
CGGGAGGTCACTCTCCCCGGGCTCTGTCCAAGTGGCGTAGGGGAGCATAGG583790.11871655244154594No Hit
TGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAGG551830.1122173300909887No Hit
CTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGTTTTC542410.1103017270076893No Hit
TCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAA510270.10376590077655946No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG67900.025.28446226
ATATAGC73650.024.4112856
TGTCGTT78100.021.92286128
TATAGCT84150.021.3656437
GCTAAGT83250.020.6485714
GTCGTTC84750.020.33440229
TTGTCGT89700.019.18910427
TCGTTCC91350.018.8399930
AATATAG111200.018.2784715
CAATATA106350.018.074114
TACAATA102900.017.760912
CGTTCCC96750.017.65202931
CTAAGTG103000.016.9950815
AGGGATC153350.016.8933642
TAAGTGC108550.016.4358416
GTTCTAT327800.016.4240381
ATAGCTG117950.015.8152928
GCTTAAA110250.015.69092441
GGGAGGT518250.015.6567983
GGAGGTC518000.015.3123794
Produced by FastQC (version 0.11.5)