FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999675

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999675
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45504769
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAAC746900.16413664246927612No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA555330.12203775828419215No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG479950.10547246157869739No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG29950.021.33140226
ATATAGC35750.019.7065096
TGTCGTT36600.017.64006228
GTCGTTC39450.016.70744129
TCGTTCC41100.015.598618530
TTGTCGT44700.015.39971727
GGGAGGT259500.015.2194323
GCGTTCG34000.015.15407415
GTCACTC252500.014.9840478
GGTCACT250500.014.97847
AGGGATC123950.014.949942
AATATAG56100.014.8895785
CAATATA53050.014.4729974
ACTCTCC266900.014.24942711
CGTTCCC45600.013.96123331
CCGCGTT38700.013.89618513
ATAGGGG181650.013.78369838
TACAATA54850.013.7516342
CGCGTTC38300.013.62962714
GGCATAG185100.013.62416735
Produced by FastQC (version 0.11.5)