FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999680

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999680
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42597256
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT4576301.0743180264944765No Hit
CCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAA689410.1618437581988849No Hit
CAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCA436270.10241739514864526No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGACCG622350.034.9885983
AGACCGC630800.034.462744
TCAGACC655300.033.6619532
CTCAGAC695900.033.2351071
GACCGCG666100.032.71325
ACCGCGT690950.031.5333796
CCGCGTT772050.028.2265857
CGCGTTC811150.026.8686548
GCGTTCT824800.026.4567249
GAACGAT844050.025.24801645
AATACGG862950.024.76219632
ATACGGA862800.024.73781433
TACGGAG862600.024.72792834
CGTTCTC888350.024.7059710
AGAACGA867350.024.65018744
CAATACG878250.024.38694831
CCCAATA886600.024.26109129
ACGGAGA882750.024.18395435
AAGAACG883950.024.17949543
CGGAGAG898700.023.72967736
Produced by FastQC (version 0.11.5)