FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999681

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999681
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42597256
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAAC1096120.2573217392218879No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA889220.20875053548050138No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG558970.13122206744960285No Hit
TCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAA508940.11947717946902495No Hit
CAGGGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAG503240.11813906510785577No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG446610.10484478155118723No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG35100.022.75429726
ATATAGC43850.019.9652236
TGTCGTT43450.018.22600428
AGGGATC154750.017.078332
TTGTCGT48650.016.8327927
GTCGTTC50050.015.77734829
TCGTTCC51500.015.37665230
AATATAG68350.014.8551525
CAATATA63650.014.5374474
GCGTTCG40150.014.23410415
TATAGCT62500.014.1876137
CAGGGAT202350.014.1393471
GGGATCA195000.014.0388793
TAGGCTA93850.013.97877910
GCTAAGT58450.013.858191514
GTCACTC236500.013.8549848
TATCCTA101200.013.7037465
CGTTCCC57250.013.67508631
ATAGGGG159250.013.63130638
ATAGCTC72000.013.55909139
Produced by FastQC (version 0.11.5)