FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999708

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999708
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50299613
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT5911411.175239658404529No Hit
CCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAA980690.1949696909198884No Hit
CAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCA553740.1100883221507092No Hit
CGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAAT503460.10009222138548064No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAGACC859750.035.3412972
CAGACCG854450.035.139153
AGACCGC860900.034.7425774
CTCAGAC907200.034.3750421
GACCGCG889150.033.6126945
ACCGCGT898850.033.2762646
CCGCGTT1021150.029.2929577
CGCGTTC1072250.027.7855728
GCGTTCT1110750.026.8589469
CGTTCTC1179100.025.37653410
GTTCTCT1290100.023.355411
CCTCTCA1285750.023.03721819
GAACGAT1273800.023.00398645
AATACGG1284700.022.97990232
ATACGGA1286000.022.94097133
TACGGAG1287750.022.87485134
TTCGACG26400.022.8400486
CCCAATA1304050.022.73345229
ACTCCCC1310000.022.61636725
CAATACG1315550.022.44601231
Produced by FastQC (version 0.11.5)