FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999709

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999709
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50299613
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAAC1605920.31927084607986944No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA1178990.23439345348442342No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG809760.16098732210921782No Hit
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGG749170.1489415037845321No Hit
CAGGGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAG729700.14507069865527594No Hit
GTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGT705450.14024958800378842No Hit
TCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAA646020.12843438775562746No Hit
CTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGTTTTC608580.12099099052710405No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGATC201900.020.1054422
GTTGTCG40450.019.74416226
ATATAGC53950.017.396676
GCGTTCG75600.016.60722415
CAGGGAT263800.016.5560741
GGGATCA246950.016.3746763
TGTCGTT51300.015.8312528
CGTTCGC80850.015.583916
CGCGTTC82600.015.52710914
CCGCGTT84250.015.32998413
TATAGCT67250.014.4581317
GGATCAT287850.014.2284164
GTCGTTC57400.014.10947429
TTCGCAC92100.013.94896818
GCTAAGT61150.013.54088914
AATATAG83100.013.520165
TCGTTCC63000.013.21212830
GTTCGCA96800.013.13241917
TTGTCGT64100.013.02086927
ATAGGGG145100.012.92924138
Produced by FastQC (version 0.11.5)