FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999717

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999717
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30765064
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAAC684690.22255438831526564No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG648520.2107975462037069No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA529400.1720783028437711No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCT431120.1401329768077193No Hit
TGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAGG394340.12817785784550942No Hit
CGGGAGGTCACTCTCCCCGGGCTCTGTCCAAGTGGCGTAGGGGAGCATAGG350800.11402544132526427No Hit
CCGGGAGGTCACTCTCCCCGGGCTCTGTCCAAGTGGCGTAGGGGAGCATAG330220.10733603544592009No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG327460.10643891395772816No Hit
CTTCTATCCCAGCGACATCGCCGTGGAGTGGGAGAGCAATGGGCAGCCGGA308350.10022732278405141No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG27250.022.86869626
ATATAGC33400.021.094186
TGTCGTT31900.019.32353628
GTCGTTC32300.018.80575629
AGTTAGG31250.018.3697684
TATAGCT38950.017.972187
TTGTCGT35050.017.90791527
TCGTTCC35050.017.8439630
GCTAAGT36950.017.53724114
GTCAGGA140050.017.2608661
AATATAG49300.016.9413875
GGGAGGT288050.016.5049463
AGGGATC99600.016.4069792
GCGTTCG31800.016.20251315
CAATATA46100.015.9683624
GGAGGTC299650.015.6716074
CGTTCGC33200.015.51856916
CGTTCCC41000.015.30923131
CTAAGTG43800.014.845615
AGCGCTC71000.014.804119
Produced by FastQC (version 0.11.5)