FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999719

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999719
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37333949
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT736100.1971663913720994No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC147050.032.7098924
CAGACCG148300.032.5869943
GACCGCG153700.031.2209425
ACCGCGT162900.029.5098676
TCAGACC187900.026.7896562
CTCAGAC208800.025.7180861
CCGCGTT238550.023.9143247
CGCGTTC261650.022.021828
GCGTTCT295250.019.5821259
CGTTCTC348150.017.0511310
CCGCGAT18500.014.865831
AATACGG389500.014.62549732
TACGGAG388350.014.53428334
ATACGGA390600.014.53409533
CAATACG400400.014.336205531
CCTCTCA418900.014.04960719
CCCAATA421350.013.83544929
ACGGAGA410300.013.77425735
GAACGAT418350.013.65857545
GTCCGCG25600.013.6104235
Produced by FastQC (version 0.11.5)