FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999721

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999721
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50231673
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG30000.015.14918126
ACCCGGC85650.013.6417925
CGCGTTC23450.012.28110414
TCACCCG101250.012.2734913
GTGGCGA98900.012.019142
TGTCGTT37450.012.01564828
GCGTTCG23600.011.91702615
CGTGGCG103750.011.8256171
CGTCGAT21650.011.74342312
TGGCGAC103050.011.6877213
GTCGTTC39700.011.50426629
GGCGACC105500.011.4163234
ATATAGC50700.011.4069036
ACGGACA99700.011.39523318
CACCCGG106100.011.3093274
CCGCGTT26650.011.06025613
AGGGATC128500.010.98500252
ATAGCTC96400.010.873839
GCGTCGA25050.010.68898311
CGTTCGC26700.010.6169816
Produced by FastQC (version 0.11.5)