FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999736

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999736
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44131136
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT6104761.3833226500219709No Hit
CCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAA587840.13320300660286652No Hit
CAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCA574990.1302912302098908No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAGACC863650.035.9713022
CTCAGAC904700.035.3676571
CAGACCG868150.035.2844353
AGACCGC881000.034.6928864
GACCGCG896050.034.0314145
ACCGCGT910650.033.5303966
CCGCGTT980550.031.1192677
CGCGTTC1007500.030.2730718
GCGTTCT1054700.028.9780189
CGTTCTC1109200.027.73675710
GAACGAT1083100.027.33709345
AATACGG1111100.026.98773632
TACGGAG1109550.026.93015734
ATACGGA1113450.026.92275433
AGAACGA1113250.026.69802944
CCCAATA1141350.026.42362829
CAATACG1139050.026.37857631
CGGAGAG1140800.026.060436
AAGAACG1143850.025.98697743
ACTCCCC1167450.025.92682625
Produced by FastQC (version 0.11.5)