FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999775

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999775
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40444130
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAAC1447350.3578640460309073No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA1056040.2611108212736929No Hit
GTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGT731790.1809384946591755No Hit
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGG675740.16707987042866296No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG655490.16207296336946797No Hit
CTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGTTTTC640310.15831963748509364No Hit
TCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAA617070.15257343896382491No Hit
CAGGGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAG603970.14933440279219753No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG533760.13197465244029233No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG33400.021.6197326
AGGGATC163100.019.9734922
ATATAGC41350.019.0551836
TGTCGTT40150.017.53676228
CAGGGAT199150.017.407991
GGGATCA195800.016.788333
GTCGTTC41900.016.64280329
TATAGCT48950.016.0507357
GGATCAT221450.014.5593214
TCGTTCC48650.014.42570930
GCTAAGT51800.014.29255614
GCGTTCG44250.014.2388215
TTGTCGT53000.013.87915627
AATATAG63200.013.8584075
GGGAGGT294100.013.6726843
CCGCGTT47250.013.66957513
CGTTCGC46150.013.60191116
CGCGTTC47700.013.53964514
CAATATA64600.013.3835864
GGAGGTC301700.013.2165174
Produced by FastQC (version 0.11.5)