FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999783

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999783
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34685715
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG22850.015.36027526
ATATAGC32000.014.1345496
CGTGGCG94250.014.1166431
GTGGCGA98550.013.3186762
TGGCGAC105100.012.7240683
CGACCCG111750.012.0014746
GCGACCC114600.011.9249275
GGCGACC112550.011.8019324
GTCACTC187650.011.6925398
GGTCACT182950.011.6482577
TATAGCT41500.011.5498117
TGTCGTT31550.011.33868728
GGGAGGT200500.011.2398873
AGGTCAC195200.011.1476296
AATATAG54750.011.1027685
CGTAGGG81000.010.94455737
GGAGGTC203250.010.86638454
GCGTTCG18800.010.77082615
CGTCGAT21150.010.74404612
CGCTGAA131950.010.69136511
Produced by FastQC (version 0.11.5)