FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999808

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999808
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39411161
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT3672080.9317360632943547No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGACCG545900.034.4352233
AGACCGC547800.034.2746244
CTCAGAC587800.033.701061
TCAGACC569150.033.684832
GACCGCG577200.032.4305425
ACCGCGT597600.031.3998746
CCGCGTT644500.029.1183727
CGCGTTC660600.028.4050148
GCGTTCT673850.027.8431769
GAACGAT679300.026.85957345
AATACGG688800.026.54718232
ATACGGA688850.026.52245733
TACGGAG689250.026.33718134
CAATACG705450.026.03556631
CCCAATA719700.025.72949429
CGTTCTC739350.025.70516810
AGAACGA713000.025.70069944
ACGGAGA709000.025.63519935
AAGAACG717300.025.5121943
CGGAGAG723800.024.986936
Produced by FastQC (version 0.11.5)