FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999810

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999810
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23356345
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG262910.11256470136915685No Hit
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG254180.10882695901263661No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT24950.023.4491846
GACCGCG26000.022.4159415
AGACCGC28550.020.8867664
CAGACCG30700.020.0103973
CGCGTAA12050.018.3074821
CCGCGTT44900.017.5974731
AACGCTA95150.017.42803223
ACGCTAC95750.017.31893224
CGCTACC96800.017.06148525
CCGTACG52700.016.1403394
AAAACGC103200.016.111721
TCCGTAC53250.016.0581473
GAAAACG100550.015.68557620
CGTACGC54350.015.6503055
AAACGCT110450.015.13605622
TTCGCGC7450.015.0999497
CCGGTAT22550.014.5745751
GTTTTCG115650.014.08523145
TCAGACC50500.013.6797892
TACGCCA63900.013.6613187
Produced by FastQC (version 0.11.5)