FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999812

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999812
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36930974
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1632190.44195693295281085No Hit
CCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAA405950.10992128179451752No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC299600.028.574854
CAGACCG302050.028.492053
GACCGCG305550.027.856415
ACCGCGT308000.027.7297926
TCAGACC332000.026.774312
CTCAGAC356450.025.9224131
CCGCGTT365350.023.3954437
CGCGTTC389400.021.9447218
TTCGACG15800.021.0753966
GCGTTCT426850.020.0404729
CGTTCTC478400.018.4538610
CGGTATA27250.016.76350220
TCGACGA22450.016.3358757
GTTCTCT565450.015.81981911
CCTCTCA566850.015.42374519
AATACGG548400.015.29214232
ATACGGA551500.015.161242533
TACGGAG544450.015.14222734
CAATACG565150.015.01807731
CCCAATA586500.014.762959529
Produced by FastQC (version 0.11.5)