FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999813

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999813
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36930974
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN799020.21635497617799085No Hit
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGG695200.18824307206194996No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA688130.1863286898417572No Hit
GTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGT422720.11446218558979787No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCCGG293200.012.90210945
TTTCTAT334300.012.3787491
TTCTATA330450.012.2481562
GGAGGTC189500.012.0673594
GGGAGGT201150.012.062823
TCTATAG330300.011.9596573
CGTGGCG77450.011.9187311
GCGTGAA327750.011.67849140
GAAGCCG327500.011.6605744
AGGGATC114850.011.5443922
AGCGTGA338500.011.36691139
GTGGCGA80100.011.3255492
ATAGGGG132500.011.10640138
TGAGCGT358150.010.75515637
GGTCACT203100.010.668877
TGGCGAC86550.010.6626943
ACTCTCC207100.010.65588511
CTATAGT375850.010.5516194
GAGCGTG369950.010.504148538
GTCACTC206300.010.470668
Produced by FastQC (version 0.11.5)