FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999825

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999825
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44647663
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA882580.19767663987250578No Hit
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGG697690.15626573780580633No Hit
CTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAAC695610.15579986795725456No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG511570.11457934539597292No Hit
GTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGT468990.10504245205398544No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG37700.017.06670626
ATATAGC50150.014.449826
TGTCGTT44300.014.37184828
GGGAGGT341550.013.9939363
AGGGATC145400.013.7488192
GGAGGTC345000.013.4562324
GCGTTCG44200.013.38836915
TATAGCT55200.013.2505967
CCGCGTT48250.013.1519113
GTCACTC339250.013.0040618
GTCGTTC50300.012.925549529
CGCGTTC47550.012.91876214
CGTTCGC47950.012.52764916
CGTGGCG78200.012.4074751
ACTCTCC362950.012.36565811
TCGTTCC51450.012.2867430
TTTCTAT399800.012.2075761
GAGGTCA385300.012.2069595
TCACTCT370850.012.1634799
TTCTATA405150.012.0020312
Produced by FastQC (version 0.11.5)