FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999827

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999827
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53399650
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA675660.12652891919703593No Hit
CTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAAC561760.10519919138046786No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG32050.015.5145126
ATATAGC51600.012.9952936
AGGGATC150450.012.6250542
TGTCGTT41300.012.47509428
GTCAGGA202250.011.4166051
GTCGTTC45950.011.40791729
TATAGCT60150.011.3724227
GCGTTCG31150.011.34063615
ATAGGGG138650.011.21241138
CGTGGCG81400.011.1418881
GGGATCA176150.011.1153343
GGGAGGT234900.011.046693
GCTAAGT50150.010.99204214
GCGTAGG78450.010.84270236
GGCGTAG79500.010.6712435
CAGGGAT205600.010.6613941
GGCATAG146950.010.62748635
GGAGGTC240150.010.6177474
TTCTATA379250.010.5983652
TTTCTAT385950.010.5717011
Produced by FastQC (version 0.11.5)