FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999837

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999837
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34514171
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG23550.017.19815326
ATATAGC36150.013.5693716
GGAGGTC213350.012.8596494
GGGAGGT228450.012.827493
TGTCGTT31750.012.68461828
GGTCACT215150.012.1322527
GCGTTCG22750.012.06581315
GTCACTC221000.011.9434538
AGGTCAC226050.011.8853336
GAGGTCA233950.011.8723335
GTCGTTC33800.011.84815129
TATAGCT41200.011.7972647
CCGCGTT24750.011.63668613
ACTCTCC230900.011.509315511
GCTAAGT37100.011.40160214
ATAGGGG148400.011.20334538
TCGTTCC36500.011.03285530
CGTGGCG57450.011.0085941
GGCATAG151800.010.86546135
TCACTCT249250.010.7613449
Produced by FastQC (version 0.11.5)