FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999856

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999856
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45398656
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG503600.11092839400355817No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG17650.020.2693446
GCCGGCT180650.016.7412661
CCGGCTT215400.015.5122652
CGGTATA26600.015.05612620
AGACCGC35100.014.9362594
CGCGTAA8950.014.5824991
TTCACGC234200.014.2864337
AACGCTA227800.014.06478423
ACGCTAC227850.014.05188424
GTTCGAC24200.014.0395035
TCACGCT238900.014.0336258
CGCTACC232000.013.6648425
TCGACGA26350.013.6627817
ACGCTCA245650.013.44622610
CGGCTTC273450.013.1901173
AAAACGC251950.012.73448721
CGTGGTT244700.012.68881141
CCGCAAT31700.012.6353641
CGACGAG24850.012.5860118
GAAAACG251900.012.567265520
Produced by FastQC (version 0.11.5)