FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999857

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999857
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45398656
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA690470.15209040549570454No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGCAA48150.011.43409244
GTTGTCG22800.011.24039126
TTCTATA252150.010.9346472
TCTATAG252200.010.6102033
GGGAGGT163350.010.4470783
TTTCTAT278450.010.3139021
CGTAACG7850.010.30328442
AGGGATC118350.010.1149452
GCGTAGG50350.010.04381536
GGCGTAG50700.010.01927435
TCGATAA60200.09.82507616
CGATAAG59950.09.79293317
ATAGGGG99000.09.734941538
GGAGGTC168800.09.6686734
CGTAGGG52150.09.65333337
TAAGTCG60050.09.43637820
GGCATAG103400.09.34724535
CTATAGT285350.09.2666114
TACGGTT40350.09.24698835
ACGCAAG60650.09.03838445
Produced by FastQC (version 0.11.5)