FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999872

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999872
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29009450
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAAC385410.13285670703856847No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA365120.12586243448255655No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG350960.12098126644938115No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG17350.016.98938626
GGTCACT208200.014.1254737
CGTGGCG88300.014.0697931
GTCACTC210600.013.974828
GTGGCGA88050.013.8540822
AGGTCAC220500.013.7455896
TGGCGAC91750.013.4182253
GGGAGGT234300.013.33309653
CGCGTTC23650.013.22380714
ATAGCTC55200.013.1636539
GAGGTCA234300.013.1222635
ACTCTCC225700.013.06970511
GCGTTCG22900.013.06750915
GGAGGTC233200.013.0387784
ATATAGC26450.013.0157786
AGGGATC78400.012.9756822
ACGCGCG20300.012.96603720
CCGCGTT24050.012.91056213
TATCCTA53900.012.8197615
ACCCGGC63350.012.6838745
Produced by FastQC (version 0.11.5)