FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999873

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999873
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29009450
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1079510.3721235666308737No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC167100.032.8425254
CAGACCG168050.032.830933
GACCGCG166450.032.727235
ACCGCGT169500.032.2291456
TCAGACC196150.029.4961382
CCGCGTT195000.028.0148077
CTCAGAC236900.026.5548441
CGCGTTC207000.026.4341018
GCGTTCT235750.023.315419
CGTTCTC283750.019.95014610
AATACGG280750.018.84139832
ATACGGA284250.018.56200433
TACGGAG284150.018.50541534
CAATACG297500.017.90915131
CCCAATA304450.017.6998629
ACGGAGA298700.017.6190335
CCGTACG57150.017.4023254
GAACGAT306300.017.28437645
CGGAGAG308200.016.9591636
CGTACGC59100.016.9041655
Produced by FastQC (version 0.11.5)