FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999874

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999874
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16575433
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT2461251.484878253255888No Hit
CCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAA342220.20646217809211984No Hit
CAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCA210580.1270434383222447No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGACCG347950.034.4626433
CTCAGAC362200.034.288931
TCAGACC354500.034.225782
AGACCGC351600.034.104784
GACCGCG365550.032.7097635
ACCGCGT375450.031.8569436
CCGCGTT415400.028.782357
CGCGTTC437050.027.3458638
GCGTTCT446250.026.8124249
CGTTCTC473600.025.37329510
GAACGAT476200.024.310445
GTTCTCT498200.024.17009511
AATACGG492950.023.76670832
ATACGGA494250.023.7087533
TACGGAG493950.023.63190334
CCCAATA498950.023.5394629
CGCGTAA5650.023.498791
CAATACG498750.023.45874431
AGAACGA496350.023.43249744
CCTCTCA506200.023.3437119
Produced by FastQC (version 0.11.5)