FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999880

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999880
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24234970
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA776110.32024384597959066No Hit
CTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAAC499230.2059957161077567No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG496750.20497240145129128No Hit
TCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAA360490.1487478631085576No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG351140.14488980180293187No Hit
CAGGGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAG325230.1341986394041338No Hit
GTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGT296990.1225460563805113No Hit
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGG283660.11704574010200962No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGGT221550.018.4356163
GGAGGTC225700.017.8576744
AGGGATC108900.017.8537982
GAGGTCA251000.016.0487125
CAGGGAT129250.015.8950091
CGTGGCG65550.015.7908561
GTCACTC246500.015.7740058
CTGGGAG193300.015.7152911
AGGTCAC254850.015.6194426
ACTCTCC250250.015.41895711
TGGGAGG187000.015.0542962
CACTCTC256750.014.95081610
GGTCACT263800.014.85937
TCACTCT263850.014.8217299
GGGATCA135350.014.6643623
GTGGCGA72400.014.1733012
CGACCCG76750.013.5745346
TGGCGAC76700.013.4669763
CCGGGAG114100.013.193531
TTCTATA352850.013.1824182
Produced by FastQC (version 0.11.5)