FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999882

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999882
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39276574
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT2064920.5257383192332407No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC348900.032.6453554
CAGACCG351100.032.492073
TCAGACC371400.031.5386962
GACCGCG361400.031.3909055
ACCGCGT364700.031.2103166
CTCAGAC461500.030.6718941
CCGCGTT393700.028.9227897
CGCGTTC406050.028.0373848
GCGTTCT493150.026.6759229
CGTTCTC542800.024.6752410
GAACGAT502750.024.53925745
ATACGGA514200.024.18450233
AATACGG516500.024.08987632
TACGGAG513300.023.9373934
AGAACGA527000.023.45698444
CAATACG538000.023.32791931
ACGGAGA528900.023.3038235
CCTCTCA550250.023.13499319
CCCAATA550700.023.12890429
CGGAGAG536950.022.74912336
Produced by FastQC (version 0.11.5)