FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999900

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999900
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23890337
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT911140.3813843228749766No Hit
CCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAA333580.13962967537879437No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG239300.10016602109882336No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC170250.031.2846684
CAGACCG174200.030.782223
GACCGCG172700.030.761515
ACCGCGT174100.030.5506846
TCAGACC187100.029.4178712
CTCAGAC215200.027.5122011
CCGCGTT215700.024.6688377
TTCGACG11850.023.5437476
CGCGTTC236550.022.2756678
GCGTTCT257400.020.506259
CGGTATA16050.020.0459620
TCGACGA16000.019.2649677
CGTTCTC285650.018.6987810
GTTCTCT335350.016.15566811
GTTCGAC16700.016.033735
CGACGAG18450.014.7555638
CCTCTCA336900.014.5920519
GTTCCTA41850.014.08548115
TCCGTAC53850.014.0821263
CCGTACG53900.014.0271974
Produced by FastQC (version 0.11.5)