FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999915

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999915
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48044745
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG2203050.4585413035286169No Hit
CTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACG683140.142188287189369No Hit
CTTCAATCCATCCAGACCATTGTGTCCCCTAATGCCTTTGAAGCCAGGAAG510560.10626760533332001No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC78850.025.3304944
CAGACCG87700.023.107583
CCGTACG86300.020.9023934
TTCGACG15000.020.8419046
TTCGGCG87000.020.74742544
CGTACGC93850.019.3159475
CTACACG96750.018.84087645
TACGCCA102550.018.1378387
TCCGTAC101400.018.0779383
TCGGCGC102050.017.55363845
CGCCGCG102400.017.40730928
AGTTGCA609200.017.12744744
TCCCGCG106900.017.10590718
CGCGCCC108300.016.88435721
GTACGCC112400.016.4882586
CGGCAGT727700.016.3968440
CAGTTGC641300.016.29509743
GTCCGTT36300.016.2463151
ACGGCAG739800.016.06612839
GCCGCGG111850.016.05878429
Produced by FastQC (version 0.11.5)