FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004999996

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004999996
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21289489
Sequences flagged as poor quality0
Sequence length35-101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG46300.017.8950941
GTACTAA42850.017.1993271
GTACTAG28350.017.1154841
GTCCTAG69650.016.6278381
TACGCGA7600.016.251092
GTATTAA61050.016.1208971
GTCTTAG54050.015.7526511
GTTCTAG70750.015.2694011
GTTCTAA65750.015.2470121
GCTCTAG84150.014.6874381
GTATATA70450.014.6846591
GTGCTAG69100.014.5740761
GTCCTAA56750.014.5192121
GGTCTAA50400.014.3503651
CTATACT50350.014.1707584
GTGTTAG64800.014.1283481
GTGCTAA61250.013.7514391
GTATAGG40000.013.0461171
GTATTAT62250.012.9422491
TCTAGAC54500.012.923863
Produced by FastQC (version 0.11.5)