FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005000001

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005000001
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20278891
Sequences flagged as poor quality0
Sequence length35-101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG43700.019.0667441
GTCCTAG70000.016.6120131
GTACTAA45550.015.9806821
GTATTAA62250.014.9294321
GTCTTAG54450.014.6297861
GTCCTAA57450.014.5033381
GTACTAG28500.014.4572011
GTCCTAT50950.014.1970961
GTGCTAG68500.013.9682611
GTTCTAG71350.013.7952971
GTTCTAA67200.013.3528331
GTCTTAA63700.013.2959361
GTCTTAT58450.013.0019991
GCTCTAG76300.012.9603221
GTACTAT41100.012.9211971
GCCCTAG74100.012.8508451
GGTCTAA44500.012.7569911
GTGCTAA56950.012.7013511
GGTCTAG53450.012.6765371
GTATATA73750.012.6014521
Produced by FastQC (version 0.11.5)