FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005000008

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005000008
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26353702
Sequences flagged as poor quality0
Sequence length35-101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG87000.018.4463481
GTATTAG59900.017.7332941
GTACTAG37250.017.159031
GTACTAA57050.016.362311
GTCTTAG69750.016.020141
GTGCTAG86750.015.2130611
GTCCTAA66650.015.1784271
GTATATA101300.015.0252871
GTGCTAA80350.014.5934681
GTTCTAG93850.014.5031271
GTATTAA87400.014.4159531
GGTCTAA62200.013.8246921
GTGTTAG86600.013.6995421
GTTCTAA88650.013.33087351
GTATAGG50850.013.2932331
GCCCTAG92800.013.2797841
CTATACT68100.013.02084
GTCTTAA85050.012.8678761
TATACTG83450.012.7177855
GCTCTAG103450.012.6683071
Produced by FastQC (version 0.11.5)