FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005000010

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005000010
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22555411
Sequences flagged as poor quality0
Sequence length35-101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG33250.018.8117681
GTCCTAG76400.017.6382961
GTATTAG48650.017.583791
GGTCTAG57300.015.6517311
GTGCTAG68450.015.3866621
GTTCTAG80600.015.34968951
GTACTAA47450.015.2175871
GCTCTAG90500.014.941051
GTATTAA73000.014.9316531
GTCTTAG65700.014.8406481
GGTCTAA56200.014.73053551
GTACTAT53200.014.6966941
GTCCTAA60150.014.4513551
GTTCTAA74800.014.3878751
GTGCTAA64350.014.1513871
GTATAGG43300.014.1268751
CTATACT57000.013.8674594
GTATATA81100.013.8372991
GTATTAT71250.013.684641
TCTAGAC58500.013.4333273
Produced by FastQC (version 0.11.5)