FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005000014

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005000014
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26267561
Sequences flagged as poor quality0
Sequence length35-101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG60250.018.0159651
GTACTAG38000.017.6714341
GTACTAA59350.016.816731
GTCCTAG87200.016.4566211
GTTCTAG94750.015.5336441
GTATTAA83850.015.5233691
GTTCTAA86900.015.1373141
GTCTTAG73700.014.7281131
GTATAGG51800.014.2066871
GTATATA102900.014.0798411
GCTCTAG100150.014.0531291
GTGCTAA77750.013.9017681
GGTCTAA63000.013.652231
GTACTAT58100.013.6161681
CTATACT72500.013.565224
GTGCTAG82450.013.4997991
GCCCTAG88800.013.3631641
GTCCTAT70400.013.1971851
GTATTAT82950.013.0857361
TCTATAC63950.013.0074573
Produced by FastQC (version 0.11.5)