FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005000016

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005000016
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24160476
Sequences flagged as poor quality0
Sequence length35-101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG36350.019.3714621
GTATTAG57450.018.2650221
GTCCTAG80650.017.8043291
GTATTAA80900.017.6355321
GTACTAA53550.017.1888141
ACGATCG13750.016.89834294-95
CTATACT63700.015.0151814
GTGCTAG76750.014.8113251
GTATATA96400.014.7999441
GTCTTAG67300.014.5659871
GTTCTAA76600.014.5399181
TATACTG78200.014.5242655
GCTCTAG93450.013.9374241
GTTCTAG84450.013.8423371
GTGCTAA68500.013.84050751
GTATAGG45100.013.7762991
GGTCTAA53850.013.7599081
GTCCTAA64800.013.7074811
TTATACT89250.013.1898064
GTATAGA59850.013.0725441
Produced by FastQC (version 0.11.5)