FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005000017

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005000017
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24160476
Sequences flagged as poor quality0
Sequence length35-101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG55400.019.1103881
GTACTAA53900.017.4217931
GTCCTAG80350.016.6135921
GTACTAG35250.016.3230881
GTGCTAG80200.015.4393621
GTTCTAG82300.015.4369211
GCGCTAG13650.015.1750911
GTCTTAG70850.015.1379641
GTCCTAA65100.014.5659111
GTATTAA82950.014.2615981
GTATATA96450.014.1744311
GTCCTAT64400.014.0094681
GTTCTAA83250.013.6019861
CTATACT68150.013.4259184
TACGCGA7550.013.3987132
GTACTAT56550.013.1866311
GTATAGG47350.013.1239691
GTACAGG141800.013.0497221
GTATAAG56550.012.7796361
GTACATA72400.012.7793541
Produced by FastQC (version 0.11.5)